APRIL 29,2015 ; WEDNESDAY
Assalamualaikum :)
Today we learn about Microbial Groups. I think I like this topic because it's quiiet easy to understand and of course because the lecture note thinner than others. Okay, let's get started! Before this, I think to indicate microbial growth, we have to look at the size of population or individual growth. But that's was wrong. Microbial growth is based on size of population. Did you know the methods of microbial reproduction? There is 5 which are binarry fission, budding, fragmentation, formation of spores and sexual reproduction.
The terms pure culture is only use in the laboratory because microorganisms do not grow as pure culture in the environment but it's exist as microbial community out there. Then, we go through requirements for development of microbial community. Firstly, physical growth requirements are light, temperature, pH, water activity and osmotic pressure. Microbes that need
light we call it as phototroph because it undergo photosynthesis to produce glucose. The lower density of light, lower phototrophs while in terms of wavelenght, it depends on photopigments. Based on the notes, I don't really understand what is meant by require/kill by light. Later I will ask Dr. Wan. Secondly,
temperature will affects microbial reproduction. There are 3 groups of microbes:
a. Mesophiles (25-40 C)
b. Psychrophiles ( <0 C but optimal at 15C)
c. Psychrotrophs (optimal at 20-30 C)
d. Thermophiles (optimal 45 C - 50 C or higher)
Next is
pH. Most bacteria can grow in pH 5-9 while fungi from pH 2-9. Very important to differentiate between Acidophiles and Alkaliphiles. Acidiphiles can survive in extreme low pH like archaebacteria while alkaliphiles prefer alkaline condition.
Water activity and osmotic pressure also important to make water movement. Water is very necessary for growth. What's difference between bacteria and fungi is in their habitat. Higher water activity for bacteria and vice versa. What is osmosis? It is diffusion of fluid through a semipermeable membrane from high concentration of water to low concentration of water. Osmotic pressure is needed to make froce to drive water movement. In addition,
|
Hypertonic --> Plasmolysis; Hypotonic --> Lysis |
Water Activity is the measurement of water that are not associated with solids or solutes (free water). The lower the solute, the higher the water activity that support more microorganisms like bacteria need 0.91 while fungi need 0.7 only. Water will flow from higher water activity to lower water activity. As an example, honey will absorb water from the air.
That's all for today. BYE :)